ISO INTERNATIONAL STANDARD 20963 First edition 2005-04-01 Soil quality Effects of pollutants on insect larvae (Oxythyrea funesta) Determination of acute toxicity Qualitedusol-Effetsdespolluantsvis-a-visdeslarvesd'insectes (Oxythyreafunesta)Détermination de latoxicité aigue Reference number ISO 20963:2005(E) @ISO2005 ISO20963:2005(E) PDF disclaimer This PDF file may contain embedded typefaces. In accordance with Adobe's licensing policy, this file may be printed or viewed but accepts no liability in this area. Adobe is a trademark of Adobe Systems Incorporated. Details of the software products used to create this PDF file can be found in the General Info relative to the file; the PDF-creation the unlikely event that a problem relating to it is found, please inform the Central Secretariat at the address given below. ISO 2005 All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying and microfilm, without permission in writing from either IsO at the address below or ISO's member body in the country of the requester. ISO copyright office Case postale 56.CH-1211Geneva 20 Tel. + 41 22 749 01 11 Fax + 41 22 749 09 47 E-mail [email protected] Web www.iso.org Published in Switzerland ii @ ISO 2005 - All rights reserved ISO20963:2005(E) Contents Page Foreword. Introductior 1 Scope 2 Normative references 3 Terms and definitions 4 Principle. 5 Test environment 6 Reagents 7 Apparatus 8 Procedure 8.1 Test design. 8.2 Preparation of testmixture 8.3 Preparationofcontrolcontainer 8.4 Food addition. 8.5 Introductionofthebiologicalmaterial. 8.6 Testconditionsandmeasurements. 8.7 Referencesubstance. 9 Expressionofresults 9.1 Calculation... 9.2 Expression ofresults.. 10 Validity of the test. 8 11 Test report... AnnexA(informative)ExampleofbreedingtechniqueforOxythyreafunesta Annex B (informative)Results of the collaborative trial carried out in France... 11 Bibliography .. 12 @ ISO 2005 - All rights reserved ili ISO20963:2005(E) Foreword IsO (the International Organization for Standardization) is a worldwide federation of national standards bodies (iSO member bodies). The work of preparing International Standards is normally carried out through ISO InternationalElectrotechnicalCommission(lEC)onallmattersofelectrotechnicalstandardization. International Standards are drafted in accordance with the rules given in the ISO/lEC Directives, Part 2. The main task of technical committees is to prepare International Standards. Draft International Standards adopted by the technical committees are circulated to the member bodies for voting. Publication as an International Standard requires approval by at least 75 % of the member bodies casting a vote. Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. IsO shall not be held responsible for identifying any or all such patent rights. ISO 20963 was prepared by Technical Committee ISO/TC 190, Soil quality, Subcommittee SC 4, Biological methods. iv @ISO 2005All rights reserved ISO20963:2005(E) Introduction This International Standard describes a method for the determination of the acute toxicity of contaminated Oxythyrea funesta has many characteristics which make it suitable for soil quality monitoring or testing effects of chemicals: aeration and drainage; the larvae of Oxythyrea funesta are tolerant to modifications of the test substrate granulometry; 一 this species can be bred under controlled conditions. ISO 2005 All rights reserved INTERNATIONAL STANDARD ISO20963:2005(E) Soil quality Effects of pollutants on insect larvae (Oxythyrea funesta) Determination of acute toxicity Scope 1 This International Standard describes a method for the determination of the effects of contaminated soils and substances on the survival of the larvae of Oxythyrea funesta. The larvae are exposed to the pollutants by cuticular and alimen