BS EN ISO 17180:2013 BSl Standards Publication Animal feeding stuffs - Determination of lysine, methionine and threonine in commercial amino acid products and premixtures (IsO 17180:2013) bsi. ..making excellence a habit: BRITISH STANDARD BSENISO17180:2013 Nationalforeword This British Standard is the UK implementation of EN ISO 17180:2013. The UK participation in its preparation was entrusted to Technical Committee AW/10, Animal feeding stuffs. A list of organizations represented onthis committee can be obtained on request to its secretary. This publication does not purport to include all the necessary provisions of a contract. Users are responsible for its correct application. @ The British Standards Institution 2013.Published by BSI Standards Limited 2013 ISBN9780580760778 ICS 65.120 Compliancewitha BritishStandard cannot confer immunity from legal obligations. This British Standard was published under the authority of the Amendments issued since publication Date Text affected EN ISO 17180 EUROPEANSTANDARD NORMEEUROPEENNE EUROPAISCHENORM April 2013 ICS 65.120 English Version Animal feeding stuffs-Determinationoflysine,methionine and threonineincommercialaminoacidproductsandpremixtures (ISO17180:2013) Aliments des animaux - Determination de la teneur en Futtermittel - Bestimmung von Lysin, Methionin und lysine, methionine et threonine dans les acides amines Threonin in handelsublichen aminosaurehaltigen Produkten industriels et les pre-melanges (ISO 17180:2013) und Vormischungen (ISO 17180:2013) This European Standard was approved by CEN on 21 March 2013 CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national status as the official versions. CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and United Kingdom. EUROPEAN COMMITTEEFOR STANDARDIZATION COMITE EUROPEEN DE NORMALISATION EUROPAISCHES KOMITEE FUR NORMUNG Management Centre:AvenueMarnix 17,B-1000 Brussels @2013CEN Allrights of exploitation in any form and by any means reserved Ref. No. EN ISO 17180:2013: E worldwide for CEN national Members. BS EN ISO17180:2013 ENISO 17180:2013 (E) Foreword This document (EN ISO 17180:2013)has been prepared by Technical Committee ISO/TC 34"Food products" in collaboration with Technical Committee CEN/TC 327"Animal feeding stuffs - Methods of sampling and analysis" the secretariat of which is held by NEN. This European Standard shall be given the status of a national standard, either by publication of an identical text or by endorsement, at the latest by October 2013, and conflicting national standards shall be withdrawn at thelatestbyOctober2013. Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights.CEN[and/orCENELEC]shall notbeheld responsibleforidentifyinganyorallsuchpatentrights According to the CEN-CENELEC Internal Regulations, the national standards organizations of the following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the United Kingdom. Endorsementnotice BSENISO17180:2013 IS0 17180:2013(E) Contents Page Foreword iv 1 Scope 1 2 Principle .1 3 Reagents and materials .1 4 .3 Apparatus 5 Procedure. 4 5.1 Preparation of samples 4 5.2 Numberofpreparedextracts 4 5.3 Extraction using dilution by weighing 4 5.4 Extractionusingvolumetric dilution 5 5.5 Chromatography 5 6 Calculation of results 5 6.1 Principle ofth