BS ISO BRITISH STANDARD 16712:2005 BS 6068-5.37:2005 Water quality Determination of acute toxicity of marine or estuarine sediment to amphipods ICS 13.080.30 BSi NO COPYING WITHOUT BSIPERMISSION EXCEPT AS PERMITTED BY COPYRIGHT LAW British Standards BS ISO 16712:2005 National foreword This British Standard reproduces verbatim IS0 16712:2005 and implements it as the UK national standard. The UK participation in its preparation was entrusted by Technical Committee BSI EH/3, Water quality, to Subcommittee EH/3/5, Biological methods,which has theresponsibilityto: aid enquirers to understand the text; present to the responsible international/European committee any enquiries on the interpretation, or proposals for change, and keep the UK interests informed; monitor related international and European developments and promulgatethem in the UK. A list of organizations represented on this subcommittee can be obtained on request to its secretary. Cross-references The British Standards which implement international publications referred to in this document may be found in the BSI Catalogue under the section entitled "International Standards Correspondence Index", or by using the"Search" facility of the BSI Electronic Catalogue or of British Standards Online. This publication does not purport to include all the necessary provisions of a contract. Users are responsible for its correct application. Compliance with a British Standard does not ofitself confer immunity from legal obligations. Summary of pages This document comprises a front cover, an inside front cover, the IsO title page, pages i to v, a blank page, pages 1 to 17 and a back cover. The BSI copyright notice displayed in this document indicates when the document was last issued. Amendmentsissuedsincepublication Licensed ( This British Standard was published under the authority Amd. No. Date Comments of the Standards Policy and Strategy Committee on 10 March 2005 BSI10March 2005 ISBN0580455920 BS IS0 16712:2005 INTERNATIONAL ISO STANDARD 16712 First edition 2005-02-15 Water quality Determination of acute toxicity of marine or estuarine sediment to amphipods QualitedeI'eau-Déterminationdelatoxiciteaiguedessédiments marins et estuariens vis-a-vis des amphipodes Reference number ISO 16712:2005(E) ISO BSIS016712:2005 ii BS IS0 16712:2005 Contents Page Scope 2 Principle 3 Test environment 3.1 Facilities 3.2 Lighting 4 Reagents and materials 4.1 Test organism 4.2 Overlying water 3 5 Apparatus 4 6 Treatment and preparation of samples 5 6.1 General 5 6.2 Control or reference sediment .... 5 6.3 Test sediment 5 6.4 Preparation of sediment samples 7 Testprocedures 6 7.1 Preparingtheexposurevessels 6 7.2 Introducing the organisms 6 7.3 Test conditions 7 7.4 Testobservationsandmeasurements 7.5Expressionofresults 8 7.6 Reburrowing capability 8 7.7 Validity of thetest 8 8 Analysis and interpretation of results 8 8.1 Data analysis 8 8.2 Non-contaminantfactors 9 9 Reference toxicant 9 10 Precision 9 11 Test report 10 AnnexA(informative)Reconstituedsaltwater 11 AnnexB(normative)Maximumlengthofamphipodspecies,optimalsalinityandtemperature ranges... 12 Annex C (informative)Precision data 13 Bibliography 14 ii BSIS016712:2005 BSI Foreword () Copy, IsO (the International Organization for Standardization) is a worldwide federation of national standards bodies Uncontrolled Electrotechnical Commission (IEC) on all matters of electrotechnical standardization. International Standards are drafted in accordance with the rules given in the ISO/lEC Directives, Part 2. Licensed Copy: sheffieldun sheffieldun, na, Sun Nov 26 03:19:44 GMT+00:00 2006, The main task of technical committees is to prepare International Standards. Draft International Standards adopted by the technical committees are circulated to the member bodies for voting. Publication as an Attention is drawn to the possibility that some of the elements of this document may be the subject of patent ISO16712waspreparedbyTechnical CommitteeISO/TC147