BS ISO 16303:2013 BS Standards Publication Water quality Determination of toxicity of fresh water sediments using Hyalella azteca bsi. ..making excellence a habit. BSISO16303:2013 BRITISH STANDARD National foreword This British Standard is the UK implementation of ISO 16303:2013. The UK participation in its preparation was entrusted to Technical Committee EH/3/5, Biological Methods. A list of organizations represented on this committee can be obtained on request to its secretary. This publication does not purport to include all the necessary provisions of a contract. Users are responsible for its correct application. The British Standards Institution 2013 Published by BSIStandards Limited 2013 ISBN9780580721823 ICS 13.060.70 Compliance with a British Standard cannot confer immunity from legal obligations. This British Standard was published under the authority of the Amendments/corrigendaissued sincepublication Date Text affected BS ISO 16303:2013 ISO 16303 INTERNATIONAL STANDARD First edition 2013-12-01 Water quality - Determination of toxicity of fresh water sediments using Hyalella azteca Qualité de I'eau - Deétermination de la toxicite des sediments d'eau doucevis-a-vis de Hyalella azteca EIE TSO ISORegeeoubey @ISO 2013 BSISO16303:2013 IS016303:2013(E) COPYRIGHTPROTECTEDDOCUMENT @ISO 2013 AHbyghtyraeansedlalestronicotherwipemifiedlaniclaindfutisgpplbbicatbpyimgyolbqnsthgintprndtiocedntnttiliaet) otineamyisowithoutprior written permission. Permission can be requested from either ISO at the address below or ISo's member body in the country of the requester. SSeoitalght officeCH-1211 Geneva 20 Tel. + 422749 0111 Fax + 41 22 749 09 47 Ww.whiso.org Published in Switzerland ii @ IS0 2013 - All rights reserved BSISO16303:2013 IS0 16303:2013(E) Contents Page Foreword iv Iptroduction. V Scope 2 Normative references 1 3 Terms and definitions .1 4 Principle .2 5 Test environment .3 5.1 Facilities .3 5.2 Lighting. 3 6 Reagents,test organisms,and materials 3 6.1 Test organism 6.2 3 Control sediment. p3od Overlviner 6.5 Reference substance .6 7 Apparatus. 6 8 Treatment and preparation of samples. 8.1 .7 General 8.2 Test sediment 7 9 Test procedure .8 9.1 Preparing the test containers .8 9.2 Introducingthe organisms 9 9.4 Kesewalcofitiexrlying.water .10 9.5 Test observations and measurements 10 10 Expression of results .10 10.1 Survival. 10 10.2 Growth. .11 11 Analysisand interpretation ofresults .11 11.1 Data analysis .11 11.2 Non-contaminantfactors 11 12 Reference substance .12 12.1 Water-only test .12 12.2 .12 Whole sedimenttest. 13 Test report. .13 AnnexA(informative)DescriptionofHyalellaazteca .14 Annex B (informative) Culturing Hyalella azteca .18 AnnexC (informative)SourcesofHyalellaazteca. .20 Annex D (normative) Procedure for preparing YcT .22 AnnexE (informative)42-daysedimentreproductiontest .23 AnnexF (informative)14-daywater-only survival-and-growthtest .24 AnnexG (informative)Performancedata .25 Bibliography 26 IS0 2013 - All rights reserved iii BS ISO 16303:2013 IS0 16303:2013(E) Foreword Iso (the International Organization for Standardization) is a worldwide federation of national standards thundiegh(IsO tecamicet codbiesfthsewofkaf ptapbeingbodyInitteresitedailhStandebjdsti soowhidhycarrtiechaital committeehasbeen establishedhastherighttoberepresented onthat committee.International organizations, governmental and non-governmental, in liaison with Iso, also take part in the work. ISO collaborates closely withthe electrotechnical standardization. International Electrotechnical Commission (IEC) on all matters of The procedures used to develop this document and those intended for its further maintenance are difeedess/cdeieshutibnotedabistafditferenwaespviferiaaccordancWith the editorial rules of the ISO/IEC Directives, Part 2 (see www.iso.org/directives). Retentionrights.dfawfsthan hetpossibthay reponsibhe fofementfrying anyehih'documaxtpa thet Pegais. subject of beintheIntroductionand/o on the Iso list of patent declara