ISO INTERNATIONAL STANDARD 16266 First edition 2006-04-15 Water quality Detection and enumeration of Pseudomonas aeruginosa Method by membrane filtration QualitedeI'eauRechercheetdenombrementdePseudomonas aeruginosaMethodeparfiltrationsurmembrane Referencenumber ISO 16266:2006(E) @ISO2006 ISO16266:2006(E) PDF disclaimer This PDF file may contain embedded typefaces. In accordance with Adobe's licensing policy, this file may be printed or viewed but shall not be edited unless the typefaces which are embedded are licensed to and installed on the computer performing the editing. In downloading this file, parties accept therein the responsibility of not infringing Adobe's licensing policy. The ISO Central Secretariat accepts no liability in this area. Adobe isatrademarkofAdobeSystems Incorporated. Details of the software products used to create this PDF file can be found in the General Info relative to the file; the PDF-creation parameters were optimized for printing. Every care has been taken to ensure that the file is suitable for use by ISO member bodies. In the unlikely event that a problem relating to it is found, please inform the Central Secretariat at the address given below. ?1SO2006 All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized in any form or by any means, Iso's memberbody in the country of the requester. ISO copyright office Case postale 56 : CH-1211 Geneva 20 Tel. + 41 22 749 01 11 Fax + 41 22 749 09 47 E-mail [email protected] Web www.iso.org Published in Switzerland ISO2006-Allrightsreserved ISO 16266:2006(E) Contents Page Foreword Introduction 1 Scope 2 Normativereferences. 3 Terms anddefinitions. 4 Principle... 5 Diluents, culture mediaand reagents.. 6 Apparatusandglassware 7 Sampling.. 8 Procedure 9 Expressionofresults 10 Test report 11 Performance data.. 8 12 Interferences 13 Qualityassurance Annex A(informative)Further information about Pseudomonas aeruginosa. AnnexB (informative)Alternativemedia... Bibliography. 12 ISO 16266:2006(E) Foreword IsO (the International Organization for Standardization) is a worldwide federation of national standards bodies International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization. International Standards are drafted in accordance with the rules given in the ISO/lEC Directives, Part 2 The main task of technical committees is to prepare International Standards. Draft International Standards adopted by the technical committees are circulated to the member bodies for voting. Publication as an International Standard requires approval by at least 75 % of the member bodies casting a vote. Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. IsO shall not be held responsible for identifying any or all such patent rights. ISO16266 was prepared byTechnical Committee ISO/TC147,Waterquality,Subcommittee SC4, Microbiologicalmethods. This International Standard is the equivalent of EN12780:2002 ISO16266:2006(E) Introduction Pseudomonas aeruginosa is an opportunistic pathogen of man that is capable of growth in water at very low Council Directive 96/7o/Ec[2]). Other bottled waters offered for sale _are also to be free of Pseudomonas andwaterfor human consumption,maysometimes betestedfor Pseudomonas aeruginosafor reasons ofpublic health.Inthesecases,itistypicaltoexamine1ooml volumes. ISO 16266:2006(E) INTERNATIONALSTANDARD Water quality Detection and enumeration of Pseudomonas aeruginosa Method by membrane filtration WARNING - Persons using this International Standard should be familiar with normal laboratory practice.This standard does not purport to address all of the safetyproblems, if any,associated with ensure compliance with any national regulatory conditions. be carried out by suitablytrained staff. 1 Scope This International Standard specifies a method for the isolation and enumeration of Pseudomonas aeruginosa in water wit