ISO INTERNATIONAL STANDARD 15473 First edition 2002-03-15 Soil quality Guidance on laboratory testing for biodegradation of organic chemicals in soil under anaerobic conditions Qualite du sol-Lignes directrices relatives aux essais en laboratoire pour labiodegradationdeproduitschimiquesorganiquesdanslesolsous conditionsanaerobies Reference number ISO 15473:2002(E) ISO @ ISO 2002 Copyright Intemational Organization for Standardization Not for Resale ISO15473:2002(E) PDF disclaimer This PDF file may contain embedded typefaces. In accordance with Adobe's licensing policy, this file may be printed or viewed but shall not be edited unless the typefaces which are embedded are licensed to and installed on the computer performing the editing. In downloading this file, parties accept therein the responsibility of not infringing Adobe's licensing policy. The ISO Central Secretariat accepts no liability in this area. Adobe is a trademark of Adobe Systems Incorporated. Details of the software products used to create this PDF file can be found in the General Info relative to the file; the PDF-creation parameters were optimized for printing. Every care has been taken to ensure that the file is suitable for use by IsO member bodies. In the unlikely event ISO 2002 All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying and microfilm, without permission in writing from either ISO at the address below or ISO's member body in the country of the requester. ISO copyright office Casepostale56.CH-1211Geneva20 Tel. + 41 22 749 01 11 Fax + 41 22 749 09 47 E-mail [email protected] Web www.iso.ch Printed in Switzerland @ ISo 2002-Allrights reserved ted without ficense from IHS Not for Resale ISO 15473:2002(E) Contents Foreword.. Introduction Scope. 7 2 Normativereferences.. : Terms and definitions. 4 Principle.. 5 Materials. Collection, handling and storage of soil. .6 6 7 Procedure 6 Expressionofresults 9 Test report. Bibliography 11 ili unde license from IHS Not for Resale ISO 15473:2002(E) Foreword ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies (isO member bodies). The work of preparing International Standards is normally carried out through IsO technical committees.Eachmemberbodyinterestedinasubjectforwhichatechnicalcommitteehasbeenestablishedhas liaison with IsO, also take part in the work. ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization. International Standards are drafted in accordance with the rules given in the ISO/lEC Directives, Part 3. The main task of technical committees is to prepare International Standards. Draft International Standards adopted by the technical committees are circulated to the member bodies for voting. Publication as an International Standard requires approval by at least 75 % of the member bodies casting a vote. Attention is drawn to the possibility that some of the elements of this International Standard may be the subject of patent rights. IsO shall not be held responsible for identifying any or all such patent rights. ISO15473wasprepared byTechnical CommitteeISO/TC190,Soil quality,Subcommittee SC4,Biological methods. @ ISO 2002 - All rights reserved onal Organization for Standardizatior ISO15473:2002(E) Introduction Organic chemicals can be introduced into the soil both intentionally and accidentally, after which they can degrade depending not only on the molecular structure of the chemical, but also on soil conditions such as temperature role in degradative processes. Iso 11266 [3] gives general guidelines for the selection and method of tests to determine the biodegradation of organicchemicalsin soils underaerobic conditions. It is necessary to have laboratory tests available to estimate the rate and extent of biodegradation under anaerobic