BS ISO 15174:2012 BSl Standards Publication Milk and milk products - Microbial coagulants - Determination of total milk- clotting activity bsi. ..making excellence a habit BS ISO 15174:2012 BRITISH STANDARD National foreword This British Standard is the UK implementation of ISO 15174:2012 The UK participation in its preparation was entrusted to Technical Committee AW/5, Chemical analysis of milk and milk products. A list of organizations represented on this committee can be obtained on request to its secretary. This publication does not purport to include all the necessary provisions of a contract. Users are responsible for its correct application. @ The British Standards Institution 2012. Published by BSI Standards Limited 2012 ISBN978 0580708794 ICS 07.100.30; 67.100.10 Compliance witha British Standard cannot confer immunity from legal obligations. This British Standard was published under the authority of the Standards Policy and Strategy Committee on 31 May 2012. Amendments issued since publication Date Text affected BS ISO 15174:2012 ISO INTERNATIONAL STANDARD 15174 IDF 176 Second edition 2012-05-15 Milk and milk products Microbial coagulants Determination of total milk- clotting activity Laitetproduitslaitiers-Coagulantsmicrobiens-Determinationde I'activite totale de coagulation du lait Reference numbers ISO 15174:2012(E) IDF 176:2012(E) [So @ISOandIDF2012 BSISO15174:2012 ISO15174:2012(E) IDF 176:2012(E) COPYRIGHTPROTECTEDDOCUMENT ISOandIDF2012 All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized in any form or by any means, address below. ISO copyright office International Dairy Federation Case postale 56 . CH-1211 Geneva 20 Silver Building:Boulevard Auguste Reyers 70/B·B-1030 Brussels Tel. + 41 22 749 01 11 Tel. + 32 2 733 98 88 Fax + 41 22 749 09 47 Fax + 32 2 733 04 13 E-mail [email protected] E-mail [email protected] Web www.iso.org Web www.fil-idf.org Published in Switzerland ii @ ISO and IDF 2012 - All rights reserved BSISO15174:2012 ISO 15174:2012(E) IDF 176:2012(E) Contents Page Foreword.. iv Foreword Introduction. 1 Scope.. 2 Principle 3 Reagents and materials. 4 Apparatus 5 Sampling. 6 Preparation of test sample ... 6.1 Liquid microbial coagulant. 6.2 Powderedmicrobial coagulant 7 7.1 Preparation of substrate... 7.2 Preparation of the microbial coagulant reference solution 7.3 Preparationof microbial coagulanttestsolution 7.4 8 Calculationandexpressionof results 8.1 Calculation... 8.2 Expressionofresults... 9 Precision.. 9.1 Interlaboratorytest. 9.2 Repeatability 9.3 Reproducibility 10 Test report. AnnexA (informative)Interlaboratorytrial.. Bibliography ISO and IDF 2012 - All rights reserved ili BS ISO15174:2012 ISO 15174:2012(E) IDF 176:2012(E) Foreword InternationalElectrotechnicalCommission(lEC)onallmattersofelectrotechnicalstandardization International Standards are drafted in accordance with the rules given in the ISO/lEC Directives, Part 2 The main task of technical committees is to prepare International Standards. Draft International Standards adopted by the technical committees are circulated to the member bodies for voting. Publication as an ISO15174liDF176 was prepared by Technical CommitteeISO/TC 34,Food products, Subcommittee SC 5 IDF. This second edition of ISO 15174liDF 176 cancels and replaces the first edition (ISO 15174liDF 176:2002) whichhasbeentechnicallyrevised iv ISO and IDF 2012 - All rights reserved BS ISO 15174:2012 ISO15174:2012(E) IDF176:2012(E) Foreword IDF (the International Dairy Federation) is a non-profit organization representing the dairy sector worldwide. IDF membership comprises National Committees in every member country as well as regional dairy the development of standard methods of analysis and sampling for milk and milk products. The main task of Standing Committees is to prepare International Standards. Draft International Standards adopted by Standing Committees are circulated to the National Committees for endo