BS ISO 15163:2012 BSl Standards Publication Milk and milk products - Calf rennet and adult bovine rennet Determination by chromatography of chymosin and bovine pepsin contents bsi. ..making excellence a habit: BS ISO15163:2012 BRITISH STANDARD National foreword This British Standard is the UK implementation of ISO 15163:2012. The UK participation in its preparation was entrusted to Technical Committee AW/5, Chemical analysis of milk and milk products. A list of organizations represented on this committee can be obtained on request to its secretary. This publication does not purport to include all the necessary provisions of a contract. Users are responsible for its correct application. @ The British Standards Institution 2012 Published by BSI Standards Limited 2012 ISBN 978 0 580 70430 7 ICS 67.100.01 Compliance with a British Standard cannot confer immunity from legal obligations. This British Standard was published under the authority of the Standards Policy and Strategy Committee on 31 July 2012. Amendments issued since publication Date Text affected BSISO15163:2012 INTERNATIONAL ISO STANDARD 15163 IDF 110 First edition 2012-05-15 MilkandmilkproductsCalfrennetand adult bovine rennet Determination by chromatography of chymosin and bovine pepsin contents Lait et produits laitiers-Presure de veau et coagulant issu debovin adulte-Determinationdesteneursenchymosine et enpepsine bovineparchromatographie Reference numbers ISO 15163:2012(E) IDF 110:2012(E) [SO @ISOandIDF2012 BS ISO 15163:2012 ISO15163:2012(E) IDF110:2012(E) COPYRIGHTPROTECTEDDOCUMENT ISO and IDF2012 All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized in any form or by any means, address below. ISO copyright office International Dairy Federation Case postale 56 . CH-1211 Geneva 20 Silver Building:Boulevard Auguste Reyers 70/BB-1030 Brussels Tel. + 41 22 749 01 11 Tel. + 32 2 733 98 88 Fax + 41 22 749 09 47 Fax + 32 2 733 04 13 E-mail [email protected] E-mail [email protected] Web www.iso.org Web www.fil-idf.org Published in Switzerland ii @ ISO and IDF 2012 - All rights reserved BSISO15163:2012 ISO15163:2012(E) IDF110:2012(E) Contents Page Foreword iv Foreword Introduction. 1 Scope. 2 Normative references... 3 Principle . 4 Reagents .. Apparatus 6 Sampling 7 Procedure 7.1 Check. 7.2 Preparationof afresh columnwithFractogel....... 7.3 Regenerationandequilibration oftheFractogel resin inthecolumn 7.4 Storage of the column with Fractogel.. 7.5 Preparation of test sample... 7.6 Analysis of the desalted rennet.... 7.7 Determination of the clotting times 8 Calculationand expression of results .. 8.1 Calculation of theactivity ofchymosin and pepsin, expressed as a percentage... 8.2 Calculation of active chymosin and active bovinepepsin, in milligrams per litre. 8.3 Expressionofresults.... 10 9 10 9.1 Interlaboratorytest. 10 9.2 Repeatability 10 9.3 Reproducibility 10 Test report... AnnexA(informative)Qualitative determination by double immunodiffusion of milk-coagulating enzymes in commercialcoagulants.. 12 AnnexB (informative)Interlaboratorytest.. Bibliography... 19 ISO and IDF 2012 - All rights reserved ili BSISO15163:2012 ISO 15163:2012(E) IDF 110:2012(E) Foreword Iso (the International Organization for Standardization) is a worldwide federation of national standards bodies (isO member bodies). The work of preparing International Standards is normally carried out through International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization. International Standards are drafted in accordance with the rules given in the IsO/lEC Directives, Part 2 adopted by the technical committees are circulated to the member bodies for voting. Publication as an Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. IsO shall not be held responsible for identifying any or all such patent rights. ISO15163liDF110waspreparedbyTechnicalCommittee