ISO INTERNATIONAL STANDARD 17734-2 Secondedition 2013-12-01 Determination of organonitrogen compounds in air using liquid chromatography and mass spectrometry - Part 2: Amines and aminoisocyanates using dibutylamine and ethyl chloroformate derivatives Determination des composes organiques azotes dans I'air par chromatographieliquideet spectrometriedemasse Partie 2: Amines et aminoisocyanates par les derives de la dibutylamineet duchloroformated'éthyle Reference number IS017734-2:2013(E) LSO Copyright International Organization for Standardization @IS02013 cense from IHS Not for Resale IS017734-2:2013(E) COPYRIGHTPROTECTEDDOCUMENT ISO2013 All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below or ISO's member body in the country of the requester. ISOcopyrightoffice Case postale 56.CH-1211 Geneva 20 Tel. +4122 749 0111 Fax +41 22 749 09 47 E-mail [email protected] Web www.iso.org Published in Switzerland @ IS0 2013 - All rights reserved No reproduction or networking permited without license from IHS Not for Resale IS017734-2:2013(E) Contents Page Foreword ..iv Introduction.. ... 1 Scope. 2 Normative references .... 3 Principle 4 Reagents and materials ..2 5 Standard solutions. .3 5.1 Reference compounds .3 5.2 Amine and deuterium-labelled amine derivatives. ..3 5.3 Aminoisocyanatederivatives .4 5.4 Thermal decomposition products of polyurethane (PUR) ..4 5.5 Stability of the amineand aminoisocyanatederivatives .4 6 Apparatus .5 7 Air sampling .7 7.1 Pre-samplinglaboratorypreparation 7.2 Pre-sampling field preparations .1 7.3 Collectionofairsamples 7.4 Blanks.. .8 7.5 Rawmaterial .8 7.6 Shipmentof samples ..8 8 Laboratory sample preparation .9 8.1 Sample sequence. .9 8.2 Work-up procedure. ..10 9 Instrumental settings. ..10 9.1 HPLC program (LC-MS) ..10 9.2 HPLC program [LC-chemiluminescent nitrogen detector (LC-CLND)] ..10 9.3 Massspectrometer. .10 10 Data handling ..11 10.1 Identification .11 10.2 Calibration curves ..11 10.3 Quantification .11 11 Determinationofperformancecharacteristics ..11 11.1 General .11 11.2 Relevant uncertainty contributions and criteria 12 11.3 Assessment of performance characteristics (following the detailed approach in Reference [12]D] 12 AnnexA (informative)Performancecharacteristics ..20 AnnexB(informative)Examples ..22 Bibliography .27 iii Sta ed without license from IHS Not for Resale